{"presentation":{"slides":[{"elements":[{"x":37.03703703703704,"y":6.479481641468682,"width":19.607843137254903,"height":6.479481641468682,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"

Principle<\/strong><\/span><\/span><\/p>\n"},"subContentId":"126cfa38-8689-42b0-a45a-b9b6e5a7266d","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":88.23529411764706,"y":62.634989200863934,"width":4.357298474945534,"height":8.639308855291576,"action":{"library":"H5P.Image 1.1","params":{"decorative":false,"contentName":"Image","file":{"path":"images\/file-657b07a3ddd26.png","mime":"image\/png","copyright":{"license":"U"},"width":128,"height":128}},"subContentId":"15807966-fdd5-47d2-a66f-b4fbdabb400d","metadata":{"contentType":"Image","license":"U","title":"Untitled Image"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":5.4409477124183,"y":17.29548596112311,"width":88.23529411764706,"height":79.91360691144709,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"

The MGIT (Mycobacteria Growth Indicator Tube) <\/strong><\/span>consists of a liquid broth medium in the form of Middlebrook 7H9 liquid media that is known to yield better recovery and faster growth of mycobacteria. The MGIT tube also contains an oxygen-quenched fluorochrome, tris 4, 7-diphenyl-1, 10-phenanthroline ruthenium chloride pentahydrate<\/strong><\/span> embedded in silicone at the bottom of the tube.<\/span><\/p>\n\n

\n\t
During bacterial growth within the tube, the free oxygen is utilized and is replaced with carbon dioxide. <\/span><\/li>\n\t
With the depletio<\/span>n of free oxygen, the fluorochrome is no longer inhibited, resulting in fluorescence within the MGIT tube when visualized under UV light. <\/span><\/li>\n\t
The intensity of fluorescence is directly proportional to the extent of oxygen depletion. <\/strong><\/span><\/span><\/li>\n\t
MGIT tubes may be incubated at 37\u00baC and read manually under a UV light or entered into an MGIT 960 instrument, where they are incubated and monitored for increasing fluorescence every 60 minutes.<\/span><\/li>\n<\/ul>\n\n
<\/p>\n"},"subContentId":"809934c9-593e-4f5a-a720-0a9e01ae84a8","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":90.41394335511983,"y":88.55291576673866,"width":5.446623093681917,"height":10.799136069114471,"action":{"library":"H5P.Image 1.1","params":{"decorative":false,"contentName":"Image","file":{"path":"images\/file-657b09d903116.png","mime":"image\/png","copyright":{"license":"U"},"width":128,"height":128}},"subContentId":"e009ddd2-8d43-4d2f-9161-192ccf05de99","metadata":{"contentType":"Image","license":"U","title":"Untitled Image"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""}],"slideBackgroundSelector":{}},{"elements":[{"x":17.42636165577342,"y":8.656177105831533,"width":76.25272331154684,"height":6.479481641468682,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"
The growth of bacteria, as well as mycobacteria, increases the fluorescence.<\/strong><\/span><\/span><\/p>\n"},"subContentId":"e4f50b3f-f2d3-4d25-b5af-c602ff74b890","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":5.443790849673203,"y":6.490734341252699,"width":9.803921568627452,"height":19.43844492440605,"action":{"library":"H5P.Image 1.1","params":{"decorative":false,"contentName":"Image","file":{"path":"images\/file-657b086ec4a58.png","mime":"image\/png","copyright":{"license":"U"},"width":128,"height":128}},"subContentId":"06682ee6-3e83-416c-b25a-d33fdd31a166","metadata":{"contentType":"Image","license":"U","title":"Untitled Image"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":23.965141612200437,"y":34.557235421166304,"width":52.287581699346404,"height":32.39740820734341,"action":{"library":"H5P.Shape 1.0","params":{"shape":{"fillColor":"rgb(239, 219, 186)","borderWidth":"0","borderStyle":"solid","borderColor":"#000","borderRadius":"0"},"type":"rectangle","line":{"borderColor":"#000000","borderWidth":"1","borderStyle":"solid"}},"subContentId":"9a07e87e-c603-47f0-b1cb-1b8df845eabd","metadata":{"contentType":"Shapes","license":"U"}},"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false},{"x":26.143790849673202,"y":36.717062634989205,"width":50.10893246187364,"height":30.23758099352052,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"
In the case of M. tuberculosis<\/em>, at the time of positivity, there are approximately 105 \u2013 106<\/strong> colony-forming units (CFU) per ml of medium.
\n
\nThe instrument declares a tube negative if it remains negative for six weeks (42 days).<\/strong><\/p>\n"},"metadata":{"contentType":"Text","license":"U","title":"Untitled Text"},"subContentId":"9a1eaaaa-c141-4385-8d26-dfe2243c6be7"},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":18.51851851851852,"y":17.278617710583152,"width":78.43137254901961,"height":15.11879049676026,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"
The detection of growth can also be visually observed by the presence of non-homogeneous light turbidity or a small granular\/flaky appearance<\/strong><\/span> in the medium.<\/p>\n"},"subContentId":"5222405d-787b-4bc0-84c0-6c20d61420dc","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""}],"slideBackgroundSelector":{}},{"elements":[{"x":6.531688453159042,"y":12.992699784017278,"width":39.21568627450981,"height":84.23326133909288,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"
Positive Cultures:<\/strong><\/span><\/span><\/p>\n\n
The system will indicate the presence of presumptive positive vials in several ways: <\/span><\/p>\n\n
\n\t
\n\t
The positive indicator lamp at the front of the drawer illuminates.<\/span><\/p>\n\t<\/li>\n\t
\n\t
The tube count for each drawer, next to the filled circle with a plus sign icon, increments in the Summary window display.<\/span><\/p>\n\t<\/li>\n\t
\n\t
When the drawer is opened, the \"remove positive tubes\" soft key appears on the screen.<\/span><\/p>\n\t<\/li>\n\t
\n\t
The audible alert sounds until the condition is acknowledged.<\/span><\/p>\n\t<\/li>\n<\/ol>\n"},"subContentId":"59a35aa1-dd92-4632-9ea8-4e2e9b73c23a","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":38.12636165577342,"y":4.319654427645788,"width":18.51851851851852,"height":5.183585313174946,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"
Notifications<\/strong><\/span><\/span><\/p>\n"},"subContentId":"9d0566ad-e5e3-47bd-98f8-74c14b043913","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":55.55555555555556,"y":12.958963282937365,"width":41.394335511982575,"height":86.39308855291577,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"
Negative Cultures:<\/span><\/strong><\/span><\/p>\n\n
Negative cultures exist as ongoing negatives (in the protocol) or out-of-protocol negatives. Notification of these conditions includes:<\/span><\/p>\n\n
\n\t
\n\t
Ongoing Negatives - In the summary region of the display, the ongoing tube count for each drawer appears next to the filled circle icon,<\/span><\/p>\n\t<\/li>\n\t
\n\t
Out-of-protocol Negatives - In the summary region of the display, the tube count for each drawer appears next to the filled circle with a minus (- )sign icon.<\/span><\/p>\n\t<\/li>\n\t
\n\t
The negative indicator light for the drawer(s) illuminates.<\/span><\/p>\n\t<\/li>\n<\/ol>\n"},"subContentId":"fca6b4ac-4bdd-4f8d-bfed-3f0a627f662d","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":45.751633986928105,"y":12.958963282937365,"width":7.625272331154684,"height":15.11879049676026,"action":{"library":"H5P.Image 1.1","params":{"decorative":false,"contentName":"Image","file":{"path":"images\/file-657b0c8e64f71.png","mime":"image\/png","copyright":{"license":"U"},"width":128,"height":128}},"subContentId":"86e1cda3-6b1f-4235-b61b-600cbb041ab1","metadata":{"contentType":"Image","license":"U","title":"Untitled Image"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":49.34444444444445,"y":28.10583153347732,"width":0.10893246187363835,"height":66.95464362850971,"action":{"library":"H5P.Shape 1.0","params":{"line":{"borderWidth":"2","borderStyle":"dotted","borderColor":"rgb(182, 182, 182)"},"type":"vertical-line","shape":{"fillColor":"#ffffff","borderColor":"#000000","borderWidth":"0","borderStyle":"solid","borderRadius":"0"}},"subContentId":"f67abee8-a81a-40b6-a3c3-6394b8e1af1a","metadata":{"contentType":"Shapes","license":"U"}},"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false}],"slideBackgroundSelector":{}}],"keywordListEnabled":true,"globalBackgroundSelector":{},"keywordListAlwaysShow":false,"keywordListAutoHide":false,"keywordListOpacity":90},"override":{"activeSurface":false,"hideSummarySlide":false,"summarySlideSolutionButton":true,"summarySlideRetryButton":true,"enablePrintButton":false,"social":{"showFacebookShare":false,"facebookShare":{"url":"@currentpageurl","quote":"I scored @score out of @maxScore on a task at @currentpageurl."},"showTwitterShare":false,"twitterShare":{"statement":"I scored @score out of @maxScore on a task at @currentpageurl.","url":"@currentpageurl","hashtags":"h5p, course"},"showGoogleShare":false,"googleShareUrl":"@currentpageurl"}},"l10n":{"slide":"Slide","score":"Score","yourScore":"Your Score","maxScore":"Max Score","total":"Total","totalScore":"Total Score","showSolutions":"Show solutions","retry":"Retry","exportAnswers":"Export text","hideKeywords":"Hide sidebar navigation menu","showKeywords":"Show sidebar navigation menu","fullscreen":"Fullscreen","exitFullscreen":"Exit fullscreen","prevSlide":"Previous slide","nextSlide":"Next slide","currentSlide":"Current slide","lastSlide":"Last slide","solutionModeTitle":"Exit solution mode","solutionModeText":"Solution Mode","summaryMultipleTaskText":"Multiple tasks","scoreMessage":"You achieved:","shareFacebook":"Share on Facebook","shareTwitter":"Share on Twitter","shareGoogle":"Share on Google+","summary":"Summary","solutionsButtonTitle":"Show comments","printTitle":"Print","printIngress":"How would you like to print this presentation?","printAllSlides":"Print all slides","printCurrentSlide":"Print current slide","noTitle":"No title","accessibilitySlideNavigationExplanation":"Use left and right arrow to change slide in that direction whenever canvas is selected.","accessibilityCanvasLabel":"Presentation canvas. Use left and right arrow to move between slides.","accessibilityProgressBarLabel":"Choose slide to display","containsNotCompleted":"@slideName contains not completed interaction","containsCompleted":"@slideName contains completed interaction","slideCount":"Slide @index of @total","containsOnlyCorrect":"@slideName only has correct answers","containsIncorrectAnswers":"@slideName has incorrect answers","shareResult":"Share Result","accessibilityTotalScore":"You got @score of @maxScore points in total","accessibilityEnteredFullscreen":"Entered fullscreen","accessibilityExitedFullscreen":"Exited fullscreen","confirmDialogHeader":"Submit your answers","confirmDialogText":"This will submit your results, do you want to continue?","confirmDialogConfirmText":"Submit and see results","slideshowNavigationLabel":"Slideshow navigation"}}

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CDST_LT: Growth detection

Target Audience

BACTEC MGIT 960 System Growth Detection

Work-up for Positive MGIT Cultures

Interpreting MGIT Results