Understand the significance of zone development or lack thereof in Wild Type (WT) and Mutation (MUT) probes in predicting drug resistance in First Line - Line Probe Assay (FL-LPA).

• Describe the Key Zones of the FL - LPA Evaluation Strip.
• Elaborate key considerations during the evaluation of an LPA strip.

Controlling contamination in LPA lab.

Describe the general instructions for performing Hybridization Using GT BLOT in LPA Lab Settings.

• Describe the test reagent kits Used for First Line (FL) and Second Line (SL) Line Probe Assay (LPA).
• Discuss the components of these kits.

• Describe the purpose of DNA extraction.
• Process of extraction of DNA from a clinical specimen and culture isolates (LJ, liquid).

Describe the uses, features and instructions for maintenance of the Twincubator as

Describe the use, components, installation and instructions for maintenance of the

Describe the uses and instructions for maintenance of the Thermocycler as a LPA Laboratory Equipment.